How does the Class II provide clean air to the working chamber?

Unidirectional HEPA filtered air cascades downward into the chamber; Air is drawn into the vented base, then travels up the rear wall plenum to the internal fan, air is discharged upstream of a full face HEPA Filter and returned to the chamber.

 

What level of air cleanliness does your incubator provide?

The Guardian® delivers an air cleanliness rating that is better than ISO Class 4 (Class 10), in accordance with ISO 14644-1 standards for controlled environment applications.  This condition is reached within 60 seconds of the Infrared Intelligent Safety Sash (IRISS) opening.

 

With the inclusion of ultraviolet (UV) light, do I still need to surface decontaminate?

UV light should not be relied upon as the sole decontaminating agent. Surface disinfection should be performed before and after every cabinet use.

 

Should I leave the UV light on at all times for optimal effectiveness?

The UV light should be turned on only when no one is in the room and the Safety Sash is completely lowered. Eyes and skin should not be exposed to this harsh light. The UV light bulb loses effectiveness over time and should be replaced when intensity drops below optimal level.

 

How often should I have my biological safety cabinet certified?

An annual certification recommended, although some institutions’ protocols require a more frequent schedule. If filters are changed or the unit is relocated the cabinet will require re-certification.

 

Where is the best location within the laboratory for a biological safety cabinet?

The ideal location is a corner area of the laboratory, away from personnel traffic, vents, doors, windows and other sources of disruptive air currents.

 

What does HEPA filter stand for?

HEPA stands for High Efficiency Particulate Arrestance Filter. Its efficiency is rated at least 99.997% efficient on particles of 0.3 microns in size.

 

Is it recommended to have more than one person working in a Biological Safety Cabinet at one time?

We do not recommend more than one user working through the front access opening of any Class II Biological Safety Cabinet.

A risk assessment should be performed by the individual most familiar with the product being considered for use within the cabinet.

When used properly, Class II Cabinets are highly effective in the reduction of laboratory-acquired infections and/or hazardous drug exposure, the protection of product from outside contaminants, and protection of cultures from cross-contamination.